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New England Nuclear Corporation gene screen plus nylon membranes
Gene Screen Plus Nylon Membranes, supplied by New England Nuclear Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+screen+plus+nylon+membranes/nylon+membrane+gene+screen+plus/us09513288-1024-13-18
Average 90 stars, based on 1 article reviews
gene screen plus nylon membranes - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Isoform-selective inhibitors and activators of PDE3 cyclic nucleotide phosphodiesterases
Article Snippet: .. PCR product samples were subjected to electrophoresis on 0.7% agarose gels, transferred to Gene Screen Plus Nylon Membranes (New England Nuclear, Boston, Mass.), cross-linked and pre-hybridized for two to three hours in QuikHyb (Stratagene). ..

Electrophoresis:

Article Title: Isoform-selective inhibitors and activators of PDE3 cyclic nucleotide phosphodiesterases
Article Snippet: .. PCR product samples were subjected to electrophoresis on 0.7% agarose gels, transferred to Gene Screen Plus Nylon Membranes (New England Nuclear, Boston, Mass.), cross-linked and pre-hybridized for two to three hours in QuikHyb (Stratagene). ..

Article Title: Salicylate-Independent Lesion Formation in <i>Arabidopsis lsd</i> Mutants
Article Snippet: In many interactions of plants with pathogens, the primary host defense reaction is accompanied by plant cell death at the site of infection.. The resulting lesions are correlated with the establishment of an inducible resistance in plants called systemic acquired resistance (SAR), for which salicylic acid (SA) accumulation is a critical signaling event in Arabidopsis and tobacco.. In Arabidopsis, the lesions simulating disease (lsd) mutants spontaneously develop lesions in the absence of pathogen infection.

Sonication:

Article Title: Methods and compositions for detecting dihydropyrimidine dehydrogenase splicing mutations
Article Snippet: .. The DNA was transferred to Gene Screen Plus nylon membranes (New England Nuclear) overnight in 0.5 M NaOH, and was fixed to the filter by baking for 90 min at 80° C. Prehybridization was done in a solution of 6×SSC, Denhardt's reagent, 0.5% NaDodSO4, and 0.2 mg/ml sonicated salmon sperm DNA at 65° C. for 3 hr, then hybridization was carried out overnight at 65° C. in the same solution containing 1.5 z 106 cpm/ml of 32P random priming-labeled human DPD, cDNA. .. After washing at 65° C. for 20 min in 2×SSC, 0.5% NaDodSO4, and 45 min in 0.1×SSC, 0.5% NaDodSO4 at 65° C., the membranes were exposed to X-ray film (Eastman Kodak Co.) at −80° C. for 30 min.

Hybridization:

Article Title: Methods and compositions for detecting dihydropyrimidine dehydrogenase splicing mutations
Article Snippet: .. The DNA was transferred to Gene Screen Plus nylon membranes (New England Nuclear) overnight in 0.5 M NaOH, and was fixed to the filter by baking for 90 min at 80° C. Prehybridization was done in a solution of 6×SSC, Denhardt's reagent, 0.5% NaDodSO4, and 0.2 mg/ml sonicated salmon sperm DNA at 65° C. for 3 hr, then hybridization was carried out overnight at 65° C. in the same solution containing 1.5 z 106 cpm/ml of 32P random priming-labeled human DPD, cDNA. .. After washing at 65° C. for 20 min in 2×SSC, 0.5% NaDodSO4, and 45 min in 0.1×SSC, 0.5% NaDodSO4 at 65° C., the membranes were exposed to X-ray film (Eastman Kodak Co.) at −80° C. for 30 min.

Clone Assay:

Article Title: Somatostatin receptor subtype 2 knockout mice are refractory to growth hormone-negative feedback on arcuate neurons.
Article Snippet: .. ES cell DNA (8 mg) from wild type AB2.1 ES cells and from four targeted clones (46, 68, 117, and 164) were digested with restriction enzyme KpnI, electrophoresed on a 0.6% agarose gel, transferred onto Gene Screen Plus nylon membranes (New England Nuclear Dupont, Boston, MA) and hybridized with the 39-probe. ..

Agarose Gel Electrophoresis:

Article Title: Somatostatin receptor subtype 2 knockout mice are refractory to growth hormone-negative feedback on arcuate neurons.
Article Snippet: .. ES cell DNA (8 mg) from wild type AB2.1 ES cells and from four targeted clones (46, 68, 117, and 164) were digested with restriction enzyme KpnI, electrophoresed on a 0.6% agarose gel, transferred onto Gene Screen Plus nylon membranes (New England Nuclear Dupont, Boston, MA) and hybridized with the 39-probe. ..



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